MERS-CoV HCoV-EMC2012滴鼻感染转基因hDPP4-Tg小鼠模型

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更新时间：2025-03-15 /

关键词:MERS-CoV HCoV-EMC2012滴鼻感染转基因hDPP4-Tg小鼠模型 /

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模型信息

中文名称:MERS-CoV HCoV-EMC2012滴鼻感染转基因hDPP4-Tg小鼠模型

英文名称:MERS-CoV HCoV-EMC2012 intransally infection transgenic hDPP4-Tg mouse model

类型:MERS-CoV感染动物模型

分级:NA

用途:用于MERS-CoV感染研究。

研制单位:病原微生物生物安全较好实验室@@http://www.natureindex.com/institution-outputs/china/state-key-laboratory-of-pathogen-and-biosecurity-amms/55010c6f140ba079548b4567

保存单位:病原微生物生物安全较好实验室@@http://www.natureindex.com/institution-outputs/china/state-key-laboratory-of-pathogen-and-biosecurity-amms/55010c6f140ba079548b4567

研究背景

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制备方法

Six-week-old female hDPP4-Tg mice27 were maintained in a pathogen-free facility and housed in cages containing sterilized feed and drinking water.r. MERS-CoV (HCoVEMC2012 strain) was propagated and titrated on Vero cells. Following intraperitoneal anesthetization with sodium pentobarbital (5 mg/kg of body weight), mice were treated intravenously (600 μg/kg, i.v.) with a monoclonal Ab (mAb) against mouse C5aR (Hycult Biotech,The Netherlands) or phosphate-buffered saline (PBS) as a sham treatment control. All mice were then intranasally inoculated with MERS-CoV (103.3 50% tissue cultureinfectious dose (TCID50)) in 20 μl Dulbecco’s modified Eagle’s medium.