MERS-CoV hCoV-EMC/2012气管途径感染恒河猴模型

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更新时间：2025-03-15 /

关键词:MERS-CoV hCoV-EMC/2012气管途径感染恒河猴模型 /

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模型信息

中文名称:MERS-CoV hCoV-EMC/2012气管途径感染恒河猴模型

英文名称:MERS-CoV hCoV-EMC/2012 intratracheally infection rhesus macaques model

类型:MERS-CoV感染动物模型

分级:NA

用途:用于MERS-CoV感染研究。

研制单位:中国疾病预防控制中心@@http://www.chinacdc.cn/en/aboutus/orc_9362/201809/t20180926_194256.html

保存单位:中国疾病预防控制中心@@http://www.chinacdc.cn/en/aboutus/orc_9362/201809/t20180926_194256.html

研究背景

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制备方法

High-dose animals were primed with 200 μg rRBD and boosted with 100-μg rRBD admixed with 1 mg of alum adjuvant. Animals received their second immunisation 8 weeks subsequent to their first. To assess long-term immunological responses, the interval between the second and third immunisations was 17 weeks. Each vaccine formulation was administered intramuscularly (i.m.).One week after the final immunisation, animals were transferred to an ABSL-3 laboratory. Following an adaptive phase of 1 week, nine immunised monkeys were anaesthetised and inoculated intratracheally with MERS-CoV of the hCoV-EMC strain at a dosage of 6.5 × 107 TCID50,diluted in 1-mL DMEM.